Changes in structure, dispersity and phase behavior of virus-like particles (VLPs) with varying antigen epitopes under chemical and mechanical stress

Virus-like particles (VLPs) are protein clusters which can be produced in recombinant expression systems and purified by centrifugation, chromatography or filtration methods. The VLP-based vaccines have been already approved for the prevention of cervical cancer, hepatitis B, hepatitis E and malaria. VLPs have a pathogen-associated structure but, contrary to attenuated viral vaccines, lack potentially infectious nucleic acids. New development approaches are increasingly focusing on the presentation of antigenic epitopes on VLPs. By the genetic or chemical fusion of antigenic epitopes to VLPs, immunogens can be produced which induce the production of specific antibodies. This can be used, for example, in cancer immunotherapy, immunotherapy of Alzheimer’s disease, and in the case of protection from infectious diseases. However, the fused antigen epitopes can fundamentally alter the structure and phase and dispersity behavior of VLPs. To study the effects of varying epitope sequences on VLPs we plan to carry out combined theoretical and experimental study.

Responsible institutes:

Institute of Engineering in Life Sciences
Section IV: Biomolecular Separation Engineering
Karlsruhe Institute of Technology
Fritz-Haber-Weg 2
76131 Karlsruhe, Germany
Institute of Nanotechnology (INT)
Karlsruhe Institute of Technology
Hermann-von-Helmholtz-Platz 1
76344 Eggenstein-Leopoldshafen, Germany
position name mail phone
Project supervisor Prof. Jürgen Hubbuch juergen.hubbuch@kit.edu +49(0)721-60847526
Postgraduate Philipp Vormittag philipp.vormittag@kit.edu +49(0)721-60846241
Project supervisor Prof. Wolfgang Wenzel wolfgang.wenzel@kit.edu +49(0)721-60826386
Project employee Dr. Priya Anand priya.anand@kit.edu +49(0)721-60822198
Project employee Prerna Sudera prerna.sudera@partner.kit.edu +49(0)6131-379324
Project employee Dr. Mariana Kozlowska mariana.kozlowska@kit.edu +49(0)721-60826448

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