Nucleation kinetics in suspension crystallization of proteins

Crystallization is an important purification step in proteins downstream processing and can be further used for formulation of crystalline products with beneficial properties, e.g. drug release properties and shelf life. Despite its high potential for the biotechnological, pharmaceutical, and food industry, preparative crystallization studies of these complex, macromolecular substances are mostly empirical. In the first part of this project, the goal is to detect the different secondary nucleation mechanisms and their influencing parameters. The second part of the research project is dedicated to a mechanistic description of the attrition of the protein crystals. By describing the secondary nucleation mechanisms and kinetics both qualitatively and quantitatively, controlled nucleation will be possible in bulk protein crystallization and, thus, the efficiency and product quality might be dramatically increased.

Responsible institute:
Institute of Thermal Process Engineering
Karlsruhe Institute of Technology (KIT)
Kaiserstr. 12, D-76131 Karlsruhe, Germany

employee  name  mail phone
Project supervisor: Prof. Matthias Kind matthias.kind@kit.edu +49(0)721-60842390
Project employee: Michael Barros Groß michael.gross@kit.edu +49(0)721-60845749

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